Nuclear Reprogramming (Record no. 262009)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04410nam a22005295i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-4939-1594-1 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20160615101629.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 141006s2015 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781493915941 |
| -- | 978-1-4939-1594-1 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1007/978-1-4939-1594-1 |
| Source of number or code | doi |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Alfaisal Main Library |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QH573-671 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSF |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI049000 |
| Source | bisacsh |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 571.6 |
| Edition number | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | Nuclear Reprogramming |
| Medium | [electronic resource] : |
| Remainder of title | Methods and Protocols / |
| Statement of responsibility, etc | edited by Nathalie Beaujean, Hélène Jammes, Alice Jouneau. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 2nd ed. 2015. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE STATEMENTS | |
| Place of production, publication, distribution, manufacture | New York, NY : |
| Name of producer, publisher, distributor, manufacturer | Springer New York : |
| -- | Imprint: Humana Press, |
| Date of production, publication, distribution, manufacture | 2015. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | XI, 285 p. 68 illus., 43 illus. in color. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content Type Term | text |
| Content Type Code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media Type Term | computer |
| Media Type Code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier Type Term | online resource |
| Carrier Type Code | cr |
| Source | rdacarrier |
| 347 ## - | |
| -- | text file |
| -- | |
| -- | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, Methods and Protocols, |
| International Standard Serial Number | 1064-3745 ; |
| Volume number/sequential designation | 1222 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Nuclear Transfer in the Mouse -- Nuclear Transfer in the Rabbit -- Nuclear Transfer in Ruminants -- Nuclear Transfer and Transgenesis in the Pig -- Embryonic Stem Cell-Somatic Cell Fusion and Post-Fusion Enucleation -- Analysis of Nuclear Reprogramming Following Nuclear Transfer to Xenopus Oocyte -- Assessing the Quality of Donor Cells: Karyotyping Methods -- Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer -- Fluorescent Immunodetection of Epigenetic Modifications on Pre-Implantation Mouse Embryos -- Visualization of Epigenetic Modifications in Preimplantation Embryos -- Live Embryo Imaging to Follow Cell Cycle and Chromosomes Stability After Nuclear Transfer -- Analysis of Nucleolar Morphology and Protein Localization as an Indicator of Nuclear Reprogramming -- Assessment of Cell Lineages and Cell Death in Blastocysts by Immunostaining -- Gene Expression Analysis in Early Embryos through Reverse Transcription Quantitative PCR (RT-qPCR) -- Studying Bovine Early Embryo Transcriptome by Microarray -- Methylation of Specific Regions: Bisulfite-Sequencing at the Single Oocyte or 2-Cell Embryo Level -- Micro Chromatin Immunoprecipitation (ChIP) from Early Mammalian Embryos -- Assessing Reprogramming by Chimera Formation and Tetraploid Complementation -- Whole-Mount In Situ Hybridization to Assess Advancement of Development and Embryo Morphology -- Genome-Wide Analysis of Methylation in Bovine Clones by Methylated DNA Immunoprecipitation (MeDIP). |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over the past several years, many technical improvements have been made to improve somatic cell nuclear transfer (SCNT) efficiency, all of which are reflected in the detailed chapters of this fully revised collection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Nuclear Reprogramming: Methods and Protocols, Second Edition will be of interest not only to cloners but also to researchers concerned with studying the development of embryos. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Life sciences. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Genetic engineering. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Cell biology. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Life Sciences. |
| 650 24 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Cell Biology. |
| 650 24 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Genetic Engineering. |
| 655 #7 - INDEX TERM--GENRE/FORM | |
| Genre/form data or focus term | Electronic books. |
| Source of term | local |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Beaujean, Nathalie. |
| Relator term | editor. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jammes, Hélène. |
| Relator term | editor. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jouneau, Alice. |
| Relator term | editor. |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer eBooks |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Display text | Printed edition: |
| International Standard Book Number | 9781493915934 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, Methods and Protocols, |
| -- | 1064-3745 ; |
| Volume number/sequential designation | 1222 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://ezproxy.alfaisal.edu/login?url=http://dx.doi.org/10.1007/978-1-4939-1594-1">http://ezproxy.alfaisal.edu/login?url=http://dx.doi.org/10.1007/978-1-4939-1594-1</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Library of Congress Classification |
| Koha item type | eBooks |
No items available.