Overview of ELISA in Relation to Other Disciplines -- Systems in ELISA -- Stages in ELISA -- Titration of Reagents -- Theoretical Considerations -- Practical Exercises -- Monoclonal Antibodies -- Validation of Diagnostic Tests for Infectious Diseases -- Charting Methods for Internal Quality Control for Competition ELISA -- Charting Methods for Internal Quality Control of Indirect ELISA -- Ruggedness and Robustness of Tests: Aspects of Kit Use and Validation -- More Advanced Statistical Methods for Quality Assurance, Test Validation, and Interpretation -- Internal Quality Control and External Quality Management of Data in Practice -- Immunochemical Techniques -- Test Questions.

The Enzyme Linked Immunosorbent Assay (ELISA) continues, as predicted, to be the predominant assay used in biomedical and agricultural research. While little has changed in the principles of the ELISAs used since the publication of The ELISA Guidebook, many technological advantages, including the increasing number of reagents available for exploitation and the larger spectrum of instruments to measure the coloured products of ELISAs, have greatly benefited the field. Other testing technologies using molecular techniques, predominantly the conventional and more and more often, real time PCR, are rapidly evolving to help diagnose and differentiate disease agents and identify substances present in exquisitely small amounts. The ELISA is still needed either alone in mass screening, such as serological assays, or used in tandem with molecular techniques to fully evaluate the molecular and antigenic nature of analytes. John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. This Edition enlarges on charting methods for assessing the indirect ELISA, ruggedness and robustness of tests, aspects of kit use and validation, and internal quality control and external quality management of data. The author describes each method in great detail to ensure experimental success and includes advice on equipment choice, maintenance, and calibration. Wherever possible, helpful written explanations are provided along with copious diagrams. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook, Second Edition offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply ELISA methodology successfully in day-to-day basic and clinical research.