This book discusses in a systematic manner the role of separation in HPLC, the types and characteristics of stationary phases and of mobile phases used in this technique, as well as other factors influencing the separation. The selection process of stationary and mobile phase for a specific separation is described as related to the physico-chemical characteristics of the molecules to be separated and of their matrix. All these subjects are discussed from the point of view of the new developments in HPLC. The book also includes a part presenting the practice of modern HPLC as necessary for applications, particularly related to the analysis of pharmaceutical and biological samples, food and beverages, environmental samples, etc. Gives a clear presentation of notions and conceptsDiscusses key parameters in HPLC separationIncludes modern developments in HPLCDescribes interrelation between various HPLC features (solvent pressure, separation, detection)Includes a large number of references.

Includes bibliographical references and index.

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Front Cover; Essentials In Modern HPLC Separations; Copyright; Dedication; Contents; Preface; Chapter1 -- Basic Information about HPLC; 1.1. INTRODUCTION TO HPLC; 1.2. MAIN TYPES OF HPLC; 1.3. PRACTICE OF HPLC; 1.4. OVERVIEW OF HPLC INSTRUMENTATION; References; Chapter2 -- Parameters that Characterize HPLC Analysis; 2.1. PARAMETERS RELATED TO HPLC SEPARATION; 2.2. EXPERIMENTAL PEAK CHARACTERISTICS IN HPLC; References; Chapter3 -- Equilibrium Types in HPLC; 3.1. PARTITION EQUILIBRIUM; 3.2. ADSORPTION EQUILIBRIUM; 3.3. EQUILIBRIA INVOLVING IONS; 3.4. EQUILIBRIUM IN SIZE-EXCLUSION PROCESSES.

3.5. THE INFLUENCE OF PH ON RETENTION EQUILIBRIA3.6. THE INFLUENCE OF TEMPERATURE ON RETENTION EQUILIBRIA; References; Chapter4 -- Intermolecular Interactions; 4.1. FORCES BETWEEN IONS AND MOLECULES; 4.2. FORCES BETWEEN MOLECULES AND A SURFACE; References; Chapter5 -- Retention Mechanisms in Different HPLC Types; 5.1. RETENTION IN REVERSED-PHASE CHROMATOGRAPHY; 5.2. RETENTION AND SEPARATION PROCESS IN ION-PAIR CHROMATOGRAPY; 5.3. RETENTION AND SEPARATION ON POLAR STATIONARY PHASES; 5.4. RETENTION PROCESS IN ION-EXCHANGE CHROMATOGRAPHY; 5.5. SEPARATION PROCESS IN CHIRAL CHROMATOGRAPHY.

5.6. RETENTION PROCESS IN SIZE-EXCLUSION CHROMATOGRAPHY5.7. RETENTION PROCESS IN OTHER CHROMATOGRAPHY TYPES; References; Chapter6 -- Stationary Phases and Their Performance; 6.1. SOLID SUPPORTS FOR STATIONARY PHASES; 6.2. REACTIONS USED FOR OBTAINING ACTIVE GROUPS OF STATIONARY PHASES; 6.3. PROPERTIES OF STATIONARY PHASES AND COLUMNS; 6.4. HYDROPHOBIC STATIONARY PHASES AND COLUMNS; 6.5. POLAR STATIONARY PHASES AND COLUMNS; 6.6. STATIONARY PHASES AND COLUMNS FOR ION-EXCHANGE, ION-MODERATED, AND LIGAND-EXCHANGE CHROMATOGRAPHY; 6.7. STATIONARY PHASES AND COLUMNS FOR CHIRAL CHROMATOGRAPHY.

6.8. STATIONARY PHASES AND COLUMNS FOR SIZE-EXCLUSION CHROMATOGRAPHY6.9. STATIONARY PHASES AND COLUMNS IN IMMUNOAFFINITY CHROMATOGRAPHY; References; Chapter7 -- Mobile Phases and Their Properties; 7.1. CHARACTERIZATION OF LIQUIDS AS SOLVENTS; 7.2. ADDITIONAL PROPERTIES OF LIQUIDS AFFECTING SEPARATION; 7.3. PROPERTIES OF THE MOBILE PHASE OF IMPORTANCE IN HPLC, NOT RELATED TO SEPARATION; 7.4. BUFFERS AND OTHER ADDITIVES IN HPLC; 7.5. GRADIENT ELUTION; 7.6. MOBILE PHASE IN REVERSED-PHASE CHROMATOGRAPHY; 7.7. MOBILE PHASE IN ION-PAIR LIQUID CHROMATOGRAPHY; 7.8. MOBILE PHASE IN HILIC AND NPC.

7.9. MOBILE PHASE IN ION-EXCHANGE AND ION-MODERATED CHROMATOGRAPHY7.10. MOBILE PHASE IN CHIRAL CHROMATOGRAPHY; 7.11. MOBILE PHASE FOR SIZE-EXCLUSION SEPARATIONS; References; Chapter8 -- Solutes in HPLC; 8.1. NATURE OF THE SOLUTE; 8.2. PARAMETERS FOR SOLUTE CHARACTERIZATION IN THE SEPARATION PROCESS; 8.3. OTHER PARAMETERS FOR SOLUTE CHARACTERIZATION; References; Chapter9 -- HPLC Analysis; 9.1. CHEMICAL NATURE OF THE ANALYTES AND THE CHOICE OF HPLC TYPE; 9.2. THE QUANTITY OF SAMPLE INJECTED FOR HPLC ANALYSIS; 9.3. ESTIMATION OF PARAMETERS DESCRIBING THE SEPARATION.

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