Structural genomics is the systematic determination of 3-dimensional structures of proteins representative of the range of protein structure and function found in nature. The goal is to build a body of structural information that will predict the structure and potential function for almost any protein from knowledge of its coding sequence. This is essential information for understanding the functioning of the human proteome, the ensemble of tens of thousands of proteins specified by the human genome. While most structural biologists pursue structures of individual proteins or protein groups, specialists in structural genomics pursue structures of proteins on a genome wide scale. This implies large scale cloning, expression and purification. One main advantage of this approach is economy of scale. Key Features *Examines the three dimensional structure of all proteins of a given organism, by experimental methods such as X-ray crystallography and NMR spectroscopy * Looks at structural genomics as a foundation of drug discovery as discovering new medicines is becoming more challenging and the pharmaceutical industry is looking to new technologies to help in this mission.

Cover; ADVANCES IN PROTEIN CHEMISTRY AND STRUCTURAL BIOLOGY; Copyright Page; Contents; Chapter 1 Preparation and Characterization of Bacterial Protein Complexesfor Structural Analysis; Abstract; I. Introduction; II. Protein Complexes of Bacteria; III. Preparation of Bacterial Protein Complexes; IV. Characterization of Protein Complexes.; V. Experimental Determination of the Structure of Protein Complexes; VI. Examples of Characterization of Bacterial Protein Complexes; VII. Future Perspectives; Acknowledgment; References.

Chapter 2 Strategies for the Cloning and Expression of Membrane ProteinsAbstract; I. Introduction; II. From Past to Present: Practical Observations Regarding Membrane Protein Expression; III. Considerations for the HTP Expression of Membrane Proteins; IV. Structural Studies of GPCRs; V. Considerations for the Expression of Ion Channels; VI. Conclusions; References; AUTHOR INDEX; SUBJECT INDEX; Color Plates.

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