Innate DNA and RNA Recognition  Methods and Protocols /  [electronic resource] : 
edited by Hans-Joachim Anders, Adriana Migliorini. 
 - XI, 195 p. 37 illus., 16 illus. in color.  online resource. 
 - Methods in Molecular Biology, Methods and Protocols,  1169  1064-3745 ; .
 - Methods in Molecular Biology, Methods and Protocols,  1169 .
<br/><br/>Detection of RNA Modifications by HPLC Analysis and Competitive ELISA -- Enzymatic Synthesis and Purification of a Defined RIG-I Ligand -- Crystallization of Mouse RIG-I ATPase Domain: In situ Proteolysis -- Isolation of RIG-I-associated RNAs from Virus Infected Cells -- Structure Modeling of Toll-like Receptors -- Nucleic Acid Recognition in Dendritic Cell -- Viral Nucleic Acid Recognition in Human Non-immune cells: In vitro Systems -- Analysis of Nucleic Acid-induced Non-immune Cell Death in Vitro -- In vitro Analysis of Nucleic Acid Recognition in B Lymphocytes -- Mapping of Optimal CD8 T Cell Epitopes -- A Modular Approach to Suppression Ass says: TLR-Ligands, Conditioned Medium and Viral Infection -- MicroRNA Methodology: Advances in miRNA-technologies -- Expression Profiling by Real-time Quantitative Polymerase Chain Reaction (RT-qPCR) -- Evaluating the Role of Nucleic Acid Antigens in Murine Models of Systemic Lupus Erythematosus -- Induction and Analysis of Nephrotoxic Serum Nephritis in Mice -- Isolation of Intratumoral Leukocytes of TLR-stimulated Tumor-bearing Mice -- Bifunctional siRNAs for Tumor Therapy. 
<br/><br/> Innate DNA and RNA Recognition: Method and Protocols presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Innate DNA and RNA Recognition: Method and Protocols provides a resource for immunologists, molecular biologists, virologists, microbiologists, and researchers studying how the innate immune system handles nucleic acids from endogenous or foreign sources. 
<br/><br/><label>ISBN: </label>9781493908820 
<br/><br/><label>Standard No.: </label>10.1007/978-1-4939-0882-0  doi 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Medicine.<br/>Human genetics.<br/>Medical genetics.<br/>Biomedicine.<br/>Human Genetics.<br/>Gene Function.
<br/><br/><label>Index Terms--Genre/Form: </label><br/>Electronic books.
<br/><br/><label>LC Class. No.: </label>RB155-155.8 QH431 
<br/><br/><label>Dewey Class. No.: </label>611.01816 599.935