TY - BOOK AU - Adams,Josephine C. ED - ScienceDirect eBooks. TI - Methods in Cell-Matrix Adhesion T2 - Methods in cell biology SN - 9780080524962 AV - QH623 .M47 2002eb PY - 2002/// CY - San Diego PB - Academic Press KW - Cell adhesion KW - Extracellular matrix KW - Cell Adhesion KW - Cell-Matrix Junctions KW - Extracellular Matrix KW - Cellules KW - Adhésivité KW - Substance fondamentale (Histologie) KW - SCIENCE KW - Life Sciences KW - Cell Biology KW - bisacsh KW - fast KW - Electronic books KW - local N1 - Includes bibliographical references and index; pt. 1. Preface and perspectives on cell-matrix adhesion -- Cell-matrix interactions: the view from the outside / Paul Bornstein -- Matrix and meaning / Martin A. Schwartz -- Cell-matrix adhesion research and the development of biotherapeutics / Roy R. Lobb -- pt. 2. Matrix methodologies -- Detection and purification of instructive extracellular matrix components with monoclonal antibody technologies / Susana G. Gil -- Isolation and purification of proteoglycans / John M. Whitelock -- Expression of recombinant matrix components using baculoviruses / Deane F. Mosher -- Heparan sulfate -- growth factor interactions / Alan C. Rapraeger -- Analysis of basement membrane self-assembly and cellular interactions with native and recombinant glycoproteins / Peter D. Yurchenco -- Preparation and analysis of synthetic multicomponent extracellular matrix / Kim S. Midwood -- Analysis of matrix dynamics by atomic force microscopy / Helen G. Hansma -- Analysis of matrix degradation / H.G. Munshi -- pt. 3. Adhesion receptor methodologies -- Expression cloning strategies for the identification of adhesion molecules / Joe W. Ramos -- Purification of integrins and characterization of integrin-associated proteins / Johannes A. Eble -- Methods for analysis of the integrin ligand binding event / Jeffrey W. Smith -- Intracellular coupling of adhesion receptors: molecular proximity measurements / Maddy Parsons -- pt. 4. Functional applications of cell-matrix adhesion in molecular cell biology -- Functional analysis of cell adhesion: quantiation of cell-matrix attachment / Steven K. Akiyama -- Measurements of glycosaminoglycan-based cell interactions / J. Kevin Langford -- Applications of adhesion molecule gene knockout cell lines / Jordan A. Kreidberg -- Flexible polyacrylamide substrata for the analysis of mechanical interactions at cell-substratum adhesions / Karen A. Beningo -- Cell migration in slice cultures / Donna J. Webb -- Application of cell adhesion to study signaling networks / Cindy K. Miranti -- Use of micropatterned adhesive surfaces for control of cell behavior / Philip LeDuc -- Adenoviral-mediated gene transfer in two-dimensional and three-dimensional cultures of mammary epithelial cells / Harriet Watkin N2 - Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users. This volume, Methods of Cell-Matrix Adhesion, contains integrated coverage on cell-matrix adhesion methods. It brings the classical methodologies and the latest techniques together in one concise volume. This coverage includes experimental protocols and their conceptual backgrou UR - http://ezproxy.alfaisal.edu/login?url=https://www.sciencedirect.com/science/bookseries/0091679X/69 ER -