Protein Nanocrystallization -- Differential Scanning Calorimetry of Proteins: an Overview and Some Recent Developments -- IR Reflectance — Absorbance Studies of Peptide Structure, Orientation, and Conformational Flexibility in Langmuir Films: Relevance for Models of Pulmonary Surfactant Action -- Two-Dimensional Infrared Correlation Spectroscopy -- NMR Spectroscopy of Large Biological Macromolecules in Solution -- Emerging Techniques in Fast Multidimensional NMR -- Principles and Application of Projected Multidimensional NMR Spectroscopy — G-matrix Fourier Transform NMR -- Protein Nanomechanics — as Studied by AFM Single-Molecule Force Spectroscopy -- Multiphoton-Excitation Fluorescence Microscopy and Membranes -- Three-Dimensional Electron Microscopy. The Coming of Age of a Versatile Structural Biology Technique.

Technical advancements are basic elements in our life. In biophysical studies, new applications and improvements in well-established techniques are being implemented every day. This book deals with advancements produced not only from a technical point of view, but also from new approaches that are being taken in the study of biophysical samples, such as nanotechniques or single-cell measurements. This book constitutes a privileged observatory for reviewing novel applications of biophysical techniques that can help the reader enter an area where the technology is progressing quickly and where a comprehensive explanation is not always to be found.