Complements: Cell death / edited by Lawrence M. Schwartz and Barbara A. Osborne. c1995.

Includes bibliographical references and index.

Isolation and assay of caspases / Srinivasa M. Srinivasula [and others] -- Cloning and analysis of Bcl-2 family genes / Enrique Cepero, Bryan W. Johnson, and Lawrence H. Boise -- Flow cytometric analysis of cell shrinkage and monovalent ions during apoptosis / Carl D. Bortner and John A. Cidlowski -- Use of flow and laser-scanning cytometry in analysis of cell death / Zbigniew Darzynkiewicz, Xun Li, and Elzbieta Bedner -- Analysis of protein transglutamylation in apoptosis / Zoltán Nemes [and others] -- Analysis of sphingomyelin and ceramide levels and the enzymes regulating their metabolism in response to cell stress / Rick T. Dobrowsky and Richard N. Kolesnick -- Cell-free systems to study apoptosis / Howard O. Fearnhead -- Role of c-Jun N-terminal kinase in apoptosis / Zheng-gang Liu [and others] -- Methods for studying pro- and antiapoptotic genes in nonimmortal cells / Mila E. McCurrach and Scott W. Lowe -- Calcium flux measurements in apoptosis / David J. McConkey and Leta K. Nutt -- Proteinase families and their inhibitors / Donald L. Mykles -- Identification and analysis of caspase substrates: proteolytic cleavage of poly(ADP-ribose)polymerase and DNA fragmentation factor 45 / Claudia Boucher [and others] -- Analysis of reactive oxygen species in cell death / Ivan Stamenkovic -- Methods for studying apoptosis and phagocytosis of apoptotic cells in drosophila tissues and cell lines / Kristin White [and others] -- Phosphatidylserine exposure and phagocytosis of apoptotic cells / Patrick Williamson, Stefan van den Eijnde, and Robert A. Schlegel -- The (Holey) study of mitochondria in apoptosis / Nigel J. Waterhouse [and others] -- In situ detection of dying cells in normal and pathological tissues / Christos Valavanis [and others] -- Model cell lines for the study of apoptosis in vitro / Christos Valavanis [and others] -- Programmed cell death assays for plants / Alan M. Jones [and others] -- Studies of apoptosis proteins in yeast / Hong Zhang and John C. Reed -- Methods to study cell death in dictyostelium discoideum / Jean-Pierre Levraud [and others] -- Methods of study of tumor necrosis factor-related ligands in apoptosis / Isabelle A. Rooney [and others].

Print version record.

Apoptosis provides a current and comprehensive collection of methods for the study of cell death. Using a diverse range of technical approaches and model systems, the chapters in this volume cover topics from the cellular and organismal to the molecular and anatomical. The methods are illustrated with user-friendly recipes and over 100 tables, halftones, and diagrams. Key Features * Current methodologies for studying cell death * Wide range of model systems * Molecular, biochemical, cellular, and genetic approaches * Complements the original Cell Death volume * Up-to-date methodology for a fast moving field * Designed with the needs of both basic scientists and clinicians in mind * Authors are leaders in their respective fields.

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