Includes bibliographical references and index.

Description based on print version record.

Front Cover; Gnotobiotics; Dedication; Gnotobiotics; Copyright; Contents; List of Contributors; Foreword; 1 -- Historical Overview; REFERENCES; 2 -- PRINCIPLES OF ESTABLISHING AND OPERATING A GNOTOBIOTIC FACILITY*; 1. INTRODUCTION; 2. INSTITUTIONAL FACTORS; 2.1 Financial Support; 2.2 Space Requirements; 2.3 Autoclave; 2.4 Personnel; 2.5 Microbiology Laboratory; 2.6 Facility Productivity and Experimental Utilization; 2.7 Facility Operation and Compliance; 3. ISOLATOR SELECTION; 3.1 Isolator Types and Construction; Chamber; Air Supply; Transfer Port; Gloves

3.2 Individual Positive Pressure Cage Systems4. STERILIZATION; 4.1 Steam; 4.2 Ionizing Radiation; 4.3 Liquid Chemical; Peracetic (Peroxyacetic, Ethaneperoxoic) Acid; Chlorine Dioxide; Other Liquid Products; Application of Liquid Sterilants; Monitoring Liquid Sterilization; 4.4 Gas and Vapor Chemical; 4.5 Dry Heat; 4.6 Filtration; 4.7 Further Information; 5. OPERATION; 5.1 Standard Operating Procedures; 5.2 Isolator Setup; Cleaning and Inspection; Leak Testing; Sterilization; 5.3 Sterilization and Entry of Supplies; Sterilizing Cylinders and Packs; Food; Water; Other Materials and Supplies

Port Sterilization5.4 Introduction and Transfer of Animals; 5.5 Working in Isolators; 5.6 Monitoring for Contamination; Sources of Contamination; Inspection and Observation; Microbiological Monitoring; Sampling; Fecal Wet Mounts and Gram Staining; Culture; Molecular Methods; Serologic Testing; Frequency of Monitoring; Summary; 5.7 Derivation of Germfree Mice; Hysterotomy and Hysterectomy; Embryo Transfer; 5.8 Establishing Defined Microbiota; Altered Schaedler Flora; Other Microbiota; 6. RESOURCES; 6.1 Recommended Reading; Gnotobiotics; Sterilization; 6.2 Online; 6.3 Manufacturers

6.4 LaboratoriesGLOSSARY; REFERENCES; 3 -- PROCEDURES FOR RODENTS*; 1. INTRODUCTION; 2. BACKGROUND AND ACKNOWLEDGMENTS; 2.1 University of Texas Southwestern Medical Center (UT Southwestern); 2.2 University of Michigan (Michigan); 2.3 University of Alabama at Birmingham (UAB); 2.4 Baylor College of Medicine (BCM); 3. GENERAL INFORMATION; 3.1 UT Southwestern; Equipment; Access; Compliance; 3.2 UAB; Equipment; Staff; General Work Practices; Access; Compliance; 3.3 BCM; Equipment; Staff; Access; Compliance; 4. STERILIZATION AND STERILIZATION MONITORING; 4.1 UT Southwestern; Autoclaving

Quality AssuranceCycles; Liquid Sterilization; 4.2 UAB; Autoclaving; General; Quality Assurance; Cycles; Liquid Sterilization; General; Sterilants; Quality Assurance; Sprayer (Atomizer); Air Hose Connection; Maintenance; 4.3 BCM; Autoclaving; Liquid Sterilization; 5. STERILIZING CYLINDERS; 5.1 UT Southwestern; Filter Medium Installation and Replacement; Equipment and Supplies; Procedure; Cylinder Maintenance; Supply Cylinders; Equipment and Supplies; Procedure; Packing Drawstring Bags; Equipment and Supplies; Procedure for Food; Procedure for Bedding

Gnotobiotics summarizes and analyzes the research conducted on the use of gnotobiotes, providing detailed information regarding actual facility operation and derivation of gnotobiotic animals.In response to the development of new tools for microbiota and microbiome analysis, the increasing recognition of the various roles of microbiota in health and disease, and the consequent expanding demand for gnotobiotic animals for microbiota/microbiome related research, this volume collates the research of this expanding field into one definitive resource.Reviews and defines gnotobiotic animal speciesAnalyzes microbiota in numerous contextsPresents detailed coverage of the protocols and operation of a gnotobiotic facility.

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