Front Matter -- Basics of Flow Cytometry / H Krishnamurthy, L Scott Cram -- Practical Considerations for Flow Cytometric Sorting of Stem Cells / Geoffrey W Osborne -- Stem Cell Analysis and Sorting Using Side Population Analysis / William Telford -- Flow Cytometry in the Study of Proliferation and Apoptosis / Michael G Ormerod, Ronald M Hamelik -- Flow Cytometric Analysis of Drug Transport and Efflux in Stem Cells / Awtar Krishan, Ronald M Hamelik -- Stem Cell Biology and Application / Swapnil Totey, Rajarshi Pal, Murali Krishna Mamidi, Vijayendran Govindasamy, Satish Totey -- Identification and Isolation of Very SmaU Embryonic-like Stem Cells from Murine and Human Specimens / Ewa K Zuba-Surma, Dong-Myung Shin, Habella Klich, Janina Ratajczak, Magda Kucia, Mariusz Z Ratajczak -- Electronic Volume of Hematopoietic Stem Cells / Siddharth Sharma, Awtar Krishan -- Hematopoietic Stem Cells: Issues in Enumeration / Michael Keeney, D Robert Sutherland -- Embryonic Stem Cells: Development and Characterization / Vijay Bhaskar R Konala, Villoo Morawala-Patell, Aparna Khanna -- Human Embryonic Stem Cells: Long-Term Culture and Cardiovascular Differentiation / Maneesha Inamdar -- Mesenchymal Stromal Cells and Their Clinical Applications / Jyoti Kode, Vivek Tanavde -- Circulating Adult Stem Cells of Hematopoietic Origin for Vascular and Neural Regeneration / Lissy K Krishnan -- Flow Cytometric Characterization of Neural Progenitors Derived from Human Pluripotent Stem Cells / Raj R Rao, Sujoy K Dhara, Shilpa Iyer, David W Machacek -- Limbal Stem Cells and Corneal Regeneration / Geeta K Vemuganti, Murali Mohan Sagar Baila, Shubha Tiwari -- Flow Cytometric Sorting of Spermatogonial Stem Cells / B S Srinag, J M Kalappurakkal, GH Mohan, H Krishnamurthy -- Breast Cancer Stem Cells / Devaveena Dey, Annapoorni Rangarajan -- Tumor Stem Cell Marker Expression in Cells from Body Cavity Fluids / Awtar Krishan, Deepti Sharma, Ronald M Hamelik -- Index.

Cover; Title Page; Copyright; Preface; Contributors; Chapter 1: Basics of Flow Cytometry; 1.1 Introduction; 1.2 Components of a Flow Cytometer; 1.3 How the Flow Cytometer Works; 1.4 Fluorescence-Activated Cell Sorting; Chapter 2: Practical Considerations for Flow Cytometric Sorting of Stem cells; 2.1 Introduction; 2.2 The Sorting Process; 2.3 Preparation of the Instrument for Stem Cell Sorting; 2.4 Cell Preparation; 2.5 Instrument Setup, Sorting, and Data Collection; 2.6 Analysis of Sorting Performance: Critical Aspects; 2.7 Conclusions.

Chapter 3: Stem Cell Analysis and Sorting Using Side Population Analysis3.1 Introduction; 3.2 Methodology; Chapter 4: Flow Cytometry in the Study of Proliferation and Apoptosis; 4.1 Introduction; 4.2 Cell Proliferation; 4.3 Apoptosis; Chapter 5: Flow Cytometric Analysis of Drug Transport and Efflux in Stem Cells; 5.1 Introduction; 5.2 Applications; 5.3 Detection of Cells with the SP Phenotype; Chapter 6: Stem Cell Biology and Application; 6.1 Introduction; 6.2 Human Embryonic Stem Cells; 6.3 Induced Pluripotent Stem Cells; 6.4 Adult Stem Cells; 6.5 Plasticity of Adult Stem Cells.

6.6 Types of Adult Stem Cells6.7 Very Small Embryoniclike Stem Cells; 6.8 Clinical Applications; Chapter 7: Identification and Isolation of Very Small Embryonic-Like Stem Cells from Murine and Human Specimens; 7.1 Introduction; 7.2 Identification and Isolation of Murine BM-derived VSELs by Classical Flow Cytometry; 7.3 Isolation of VSELs from Human Umbilical Cord Blood by Classical Flow Cytometry; 7.4 Identification of Murine and Human VSELs by Imaging Cytometry; Chapter 8: Electronic Volume of Hematopoietic Stem Cells; 8.1 Introduction; 8.2 Applications; 8.3 Materials and Methods.

8.4 Results8.5 Conclusions; 8.6 Artifacts, Precautions, and Critical Aspects; Chapter 9: Hematopoietic stem Cells: Issues in Enumeration; 9.1 Introduction; 9.2 Hematopoietic Stem or Progenitor Cells and the CD34 Antigen; 9.3 CD45 Antigen: Structural Considerations and Choice of Conjugates; 9.4 Multiparameter Cytometry and Sequential Boolean Gating: The ISHAGE Protocol; 9.5 Immunological Characterization of CD34+ Stem Cells; 9.6 Conclusions; 9.7 The ISHAGE Protocol: Flow Cytometric Enumeration of CD34+ Hematopoietic Progenitor Cells.

Chapter 10: Embryonic Stem Cells: Development and Characterization10.1 Introduction and Background; 10.2 Establishment of a Human Embryonic Stem Cell Line; 10.3 Characterization of Human Embryonic Stem Cell Lines; Chapter 11: Human Embryonic Stem Cells: Long-Term Culture and Cardiovascular Differentiation; 11.1 Introduction; 11.2 Cardiovascular Development; 11.3 Overview of Protocols; 11.4 Protocols; 11.5 Differentiation of hES Cells into Cardiovascular Lineages; Chapter 12: Mesenchymal Stromal Cells and Their Clinical Applications; 12.1 Introduction; 12.2 Applications.

12.3 Materials and Reagents.

A much-needed primer on the use of laser flow cytometry for stem cell analysisLaser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation, and apoptosis. However, no resources specifically address the use of this methodology for the study of stem cells; this is especially important as stem cell analysis involves specialized methods and staining procedures based on specific characteristics such as marker expression, cell size, drug transport, and efflux of the stem cells. Now, this book reviews these procedures, discusses the scie.

Includes bibliographical references and index.

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