The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike. Highlights * Cloning * Expression systems * Signal transduction * Structure-function techniques * Antireceptor antibodies * Regulation * 3-D receptor modeling and computational probing.

Receptor Cloning: -- S.C. Sealfon, Approaches to Receptor Cloning. -- D.I. Schuster and R.B. Murphy, Purification of Receptors. -- M.J. Walsh, Protein and Peptide Microsequencing: Applications in Neuroscience and Receptor Research. -- R.D. Zuhlke, H.-J. Zhang, and R.H. Joho, Xenopus Oocytes: A System for Expression Cloning and Structure--Function Studies of Ion Channels and Receptors. -- D.K. Grandy, Q.-Y. Zhou, C. Bouvier, C. Saez, and J.R. Bunzow, Cloning of G Protein-Coupled Opioid Receptors Using Degenerate PCR and Low Stringency Homology Screening. -- M. Tsutsumi and B. Gillo, Hybrid Arrest Screening in Oocytes. -- E.F. Kirkness and J.C. Venter, Receptor Cloning: High-Throughput Sequencing of cDNA Tags for Identification of Novel Genes. -- Expression and Characterization: -- R.P. Millar, J. Davidson, C. Flanagan, and I. Wakefield, Ligand Binding and Second Messenger Assays for Cloned Gq/G11-Coupled Neuropeptide Receptors: The GnRH Receptor. -- R.L. Neve and K.A. Neve, Receptor Expression in Mammalian Cells. M. Cascio, Baculovirus Expression of Receptors and Channels. -- J.A. Salon and J.C. Owicki, Real-Time Measurements of Receptor Activity: Applications of Microphysiometric Techniques to Receptor Biology. -- K.A. Neve, M.P. Rosser, and D.L. Barber, Regulation of Na⁺-H⁺ Exchange by G Protein-Coupled Receptors. -- P.J.S. Stork, A. Misra-Press, and M.-G. Pan, Receptor-Activated Tyrosine Phosphatases: Activity Assays and Molecular Cloning. -- Studies of Functional Domains of Receptor and Channels: -- T.M. Fong, M.R. Candelore, and C.D. Strader, Site-Directed Mutagenesis. -- S. Suryanarayana and B.K. Kobilka, Receptor Chimers. -- D. Palm, G. Munch, and D. Malek, Mapping G Protein Coupling Domains by Site-Specific Peptides. -- C.J.L. Carruthers and T.P. Sakmar, Synthesis and Expression of Synthetic Genes: Applications to Structure-Function Studies of Receptors. -- J. Hoebeke, J.-G. Guillet, and A.D. Strosberg, Use of Receptors Expressed in Escherichia coli to Study Autoimmunity against G Protein-Coupled Membrane Proteins. -- J.A. Ballesteros and H. Weinstein, Integrated Methods for the Construction of Three Dimensional Models and Computational Probing of Structure-Function Relations in G-Protein Coupled Receptors. -- Localization and Regulation: -- B.J. Ciliax, C. Heilman, S. Edmunds, S.M. Hersch, and A.I. Levey, Anti-fusion Protein Antibodies Specific for Receptor Subtypes. -- M.A. Ariano and D.R. Sibley, Development of Antireceptor Antibodies Using Synthetic Peptides. -- M. Jakubowski, Receptor mRNA Measurement by Multiplex Nuclease Protection Assay. -- M. Shih, C.M. Moxham, and C.C. Malbon, Antisense DNA/RNA-Based Strategies to Analysis of Signal Transduction via G Proteins. -- Index.

Includes bibliographical references and index.

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