Intracellular Parcel Service: Current Issues in Intracellular Membrane Trafficking -- In vitro Analysis of the Mitochondrial Preprotein Import Machinery Using Recombinant Precursor Polypeptides -- Import of Proteins into Isolated Yeast Mitochondria -- Evaluation of Unconventional Protein Secretion in Saccharomyces cerevisiae -- Fractionation of Plasmodium-infected Human Red Blood Cells to Study Protein Trafficking -- Investigating Signalling Processes in Membrane Trafficking -- Recruitment of Coat Proteins to Liposomes and Peptidoliposomes -- A β-lactamase Based Assay to Measure Surface Expression of Membrane Proteins -- Cell-free Reconstitution of Multivesicular Body (MVB) Cargo Sorting -- The Analysis of the Biogenesis of Lipid Droplets by Examining Rab40c Associating With Lipid Droplets -- Analysis of Conventional and Unconventional Trafficking of CFTR and Other Membrane Proteins -- Assessing Mammalian Autophagy -- Expression of Functional Myc-tagged Conserved Oligomeric Golgi (COG) Subcomplexes in Mammalian Cells -- Molecular and Cellular Characterization of GCC185, a Tethering Protein of the trans-Golgi Network -- Visualizing Toll-like Receptor-dependent Phagosomal Dynamics in Murine Dendritic Cells Using Live Cell Microscopy -- Understanding of Complex Protein Interactions with Respect to Anchorage Independence -- Application of Flow Cytometry to Analyse Intracellular Location and Trafficking of Cargo in Cell Populations -- Approaches to Analyze the Role of Rab GTPases in Endocytic Trafficking of Epidermal Growth Factor Receptor (EGFR) -- Does Super Resolution Fluorescence Microscopy Obsolete Previous Microscopic Approaches to Protein Co-localization? -- Bimolecular Fluorescence Complementation (BiFC) Technique in Yeast Saccharomyces cerevisiae and Mammalian Cells -- Microscopic and Spectroscopic Techniques to Investigate Lipid Droplet Formation and Turnover in Yeast -- Image-based Identification of Nuclear Export Inhibitors From Natural Products -- Correlative Video-light-electron Microscopy of Mobile Organelles -- Live Cell Imaging of Endosomal Trafficking in Fungi -- Quantitative Analysis of Transferrin Cycling by Automated Fluorescence Microscopy -- Identification of Factors Regulating MET Receptor Endocytosis by High Throughput siRNA Screening -- Large-scale Analysis of Membrane Transport in Yeast Using Invertase Reporters -- RNAi Screens for Genes Involved in Golgi Glycosylation -- Proteomics Analyses of an Unique Bi-lobed Structure in Trypanosoma brucei -- Application of the Proximity-dependent Assay and Fluorescence Imaging Approaches to Study Viral Entry Pathways.

This second edition explores membrane trafficking research that has been at the frontier of cell and molecular biology. In the past 20 years, this field has witnessed a remarkable convergence and synergism from information gained using genetic approaches in yeast cells, and biochemical and molecular approaches in mammalian cells. This volume contains updated chapters and the inclusion of a large number of new chapters, and is divided into three parts. Part I includes biochemical and molecular genetics approaches and methods used in analysing membrane traffic, in both yeast and mammalian cell models. Part II focuses on imaging and microscopy approaches and techniques, while Part III highlights the Omics-type approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and resourceful, Membrane Trafficking, Second Edition, is a valuable resource for both experienced researchers, graduate students, and junior scientists in the field of membrane trafficking.