Includes bibliographical references and index.

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In recent years, a number of academic and commercial software packages and databases have been developed for the analysis and screening of biological data; however, the usability of these data is compromised by so-called novel genes to which no biological function is assigned. Annotating new genes outlines an approach to the analysis of evolutionary-conserved, heart-enriched genes with unknown functions, offering a step-by-step description of the procedure from screening to validation. The book begins by offering an introduction to the databases and software available, before moving on to cover programming guidelines, including a specific case study on the use of C-It for in silico screening. The second half of the book offers a step-by-step guide to experimental validation concepts and procedures, as well as an overview of additional potential applications of this approach in the field of stem cells and tissue regeneration, before a concluding chapter summarises the concepts and theories presented. Focuses not only on screening but also on biological validationsProvides details of databases and software (web interface) products for biologists with minimal computation skillsOffers a step-by-step outline of the procedure involved.

Cover; Annotating new genes: From in silico screening to experimental validation; Copyright; Contents; List of figures and tables; Acknowledgements; About the author; 1 Introduction; 2 Databases and software to make your research life easier; 2.1 Introduction; 2.2 Primary databases; 2.3 Secondary databases; 2.4 Datasets to keep an eye out for; 2.5 Conclusion; References; 3 How to program like a bioinformatician?; 3.1 Introduction; 3.2 Setting up an environment; 3.3 Writing Perl scripts; 3.4 Conclusion; References.

4 Case study: C-It, knowledge database for screening evolutionarily conserved, tissue-enriched, uncharacterized genes4.1 Introduction; 4.2 Concept of in silico screening; 4.3 Case study: C-It; 4.4 Conclusion; References; 5 Experimental validation: concepts and practice; 5.1 Introduction; 5.2 Proposed experimental strategy; 5.3 Step 1: validation of expression patterns; 5.4 Step 2: phenotypic analysis; 5.5 Step 3: molecular interactions; 5.6 Other experiments; 5.7 Conclusion; References; 6 Further application: adult stem cells and tissue regenerations; 6.1 Introduction.

6.2 Cell types in heart and their lineages6.3 Current status of cardiac stem cells; 6.4. Conclusion; References; 7 Conclusion; Reference; Index.

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