000			04799nam a22005535i 4500
001			978-1-59259-958-5
003			DE-He213
005			20160614135352.0
007			cr nn 008mamaa
008			100301s2006 xxu| s |||| 0|eng d
020			_a9781592599585 _9978-1-59259-958-5
024	7		_a10.1385/1592599583 _2doi
049			_aAlfaisal Main Library
050		4	_aQK600-638
072		7	_aPSQ _2bicssc
072		7	_aPSAK _2bicssc
072		7	_aSCI086000 _2bisacsh
072		7	_aSCI029000 _2bisacsh
082	0	4	_a579.135 _223
245	1	0	_aYeast Protocol _h[electronic resource] / _cedited by Wei Xiao.
250			_aSecond Edition.
264		1	_aTotowa, NJ : _bHumana Press : _bImprint: Humana Press, _c2006.
300			_aXII, 392 p. 104 illus. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
490	1		_aMethods in Molecular Biology, _x1064-3745 ; _v313
505	0		_aBasic Investigations in Saccharomyces cerevisiae -- Isolation of Nucleic Acids -- Purification of Yeast Peroxisomes -- Isolation of Yeast Plasma Membranes -- Isolation of Yeast Mitochondria -- Extraction of Yeast Lipids -- Two-Dimensional Gel Electrophoresis of Total Yeast Proteins -- Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes -- Analysis of Yeast Lipids -- Yeast Fluorescence Microscopy -- Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombe -- Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method -- Mutagenesis -- Gene Disruption in the Budding Yeast Saccharomyces cerevisiae -- Inducible Degron and Its Application to Creating Conditional Mutants -- Synthetic Lethal Screen -- Synthetic Genetic Array Analysis in Saccharomyces cerevisiae -- Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates -- Chromatin Assembly in a Crude Fraction From Yeast Cells -- Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding Yeast -- Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome Positioning -- Study of Transcriptional Regulation Using a Reporter Gene Assay -- Assessing Telomeric Phenotypes -- Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast -- Production of Heterologous Proteins in Yeast With the Aid of the Hsp150? Carrier -- Yeast Two-Hybrid System Screening -- Import of Precursor Proteins Into Isolated Yeast Mitochondria.
520			_aIn this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred during the last decade to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research. Yeast Protocols, Second Edition contains 27 optimized and readily reproducible techniques suitable for both yeast researchers and investigators who wish to use yeast to study their favorite genes from other organisms. The authors provide detailed methods for the isolation of subcellular components, including organelles and macromolecules; for the basic cellular and molecular analysis specific for yeast cells; and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Yeast Protocols, Second Edition offers researchers a collection of traditional and novel protocols essential for studying yeasts, as well as genes from other organisms.
650		0	_aLife sciences.
650		0	_aBiochemistry.
650		0	_aCell biology.
650		0	_aMycology.
650	1	4	_aLife Sciences.
650	2	4	_aMycology.
650	2	4	_aCell Biology.
650	2	4	_aBiochemistry, general.
655		7	_aElectronic books. _2local
700	1		_aXiao, Wei. _eeditor.
710	2		_aSpringerLink (Online service)
773	0		_tSpringer eBooks
776	0	8	_iPrinted edition: _z9781588294371
830		0	_aMethods in Molecular Biology, _x1064-3745 ; _v313
856	4	0	_uhttp://ezproxy.alfaisal.edu/login?url=http://dx.doi.org/10.1385/1592599583
912			_aZDB-2-PRO
942			_2lcc _cEBOOKS
999			_c247307 _d247307