000			05378nam a22005055i 4500
001			978-1-59745-130-7
003			DE-He213
005			20160614135353.0
007			cr nn 008mamaa
008			100301s2006 xxu| s |||| 0|eng d
020			_a9781597451307 _9978-1-59745-130-7
024	7		_a10.1385/1597451304 _2doi
049			_aAlfaisal Main Library
050		4	_aQK1-989
072		7	_aPST _2bicssc
072		7	_aSCI011000 _2bisacsh
072		7	_aNAT026000 _2bisacsh
082	0	4	_a580 _223
245	1	0	_aAgrobacterium Protocols _h[electronic resource] / _cedited by Kan Wang.
250			_aSecond Edition.
264		1	_aTotowa, NJ : _bHumana Press, _c2006.
300			_aXXII, 484 p. 89 illus., 6 illus. in color. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
490	1		_aMethods in Molecular Biology, _x1064-3745 ; _v343
505	0		_aAGROBACTERIUM HANDLING -- Culture and Maintenance of Agrobacterium Strains -- Binary Vectors and Super-binary Vectors -- Three Methods for the Introduction of Foreign DNA into Agrobacterium -- Integration of Genes into the Chromosome of Agrobacterium tumefaciens C58 -- Nucleic Acid Extraction from Agrobacterium Strains -- Agrobacterium Virulence Gene Induction -- Model Plants -- Arabidopsis thaliana Floral Dip Transformation Method -- Agrobacterium Transformation of Arabidopsis thaliana Roots -- Medicago truncatula Transformation Using Leaf Explants -- Medicago truncatula Transformation Using Cotyledon Explants -- Medicago truncatula Transformation Using Root Explants -- Nicotiana (Nicotiana tobaccum, Nicotiana benthamiana) -- Generation of Composite Plants Using Agrobacterium rhizogenes -- Cereal Crops -- Barley (Hordeum vulgare L.) -- Maize (Zea mays L.) -- Indica Rice (Oryza sativa, BR29 and IR64) -- Japonica Rice Varieties (Oryza sativa, Nipponbare, and Others) -- Rye (Secale cereale L.) -- Sorghum (Sorghum bicolor L.) -- Wheat (Triticum aestivum L.) -- Industrial Plants -- Canola (Brassica napus L.) -- Cotton (Gossypium hirsutum L.) -- Indian Mustard [Brassica juncea (L.) Czern.] -- Sunflower (Helianthus annuus L.) -- Legume Plants -- Alfalfa (Medicago sativa L.) -- Chickpea (Cicer arietinum L.) -- Clovers (Trifolium spp.) -- Peas (Pisum sativum L.) -- Peanut (Arachis hypogaea L.) -- Pigeonpea (Cajanus cajn (L.) Millsp.) -- Red Clover (Trifolium pratense) -- Soybean (Glycine max) Transformation Using Mature Cotyledonary Node Explants -- Soybean (Glycine max) Transformation Using Immature Cotyledon Explants -- Tepary Bean (Phaseolus acutifolius) -- Vegetable Plants -- Brassica oleracea -- Cucumber (Cucumis sativus L.) -- Eggplant (Solanum melongena L.) -- Lettuce (Lactuca sativa L.) -- Tomato (Lycopersicum esculentum).
520			_aRapid changes and significant progress have been made in the use of Agrobacterium to genetically transform plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Second Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail their best techniques for delivering DNA to plant cells and permanently altering their genomes. Volume 1 details the most updated techniques available for twenty-six plant species drawn from cereal crops, industrial plants, legume plants, and vegetable plants, and presents various methods for introducing DNA into three major model plant species, Arabidopsis thaliana, Medicago truncatula, and Nicotiana. The authors also outline the basic methods in Agrobacterium manipulation and strategies for vector construction, major components of plant transformation that are often neglected by many plant biologists. Volume 2 contains another thirty-three proven techniques for root plants, turf grasses, woody species, tropic plants, nuts and fruits, ornamental plants, and medicinal plants. Additional chapters provide methods for introducing DNA into non-plant species, such as bacteria, fungi, algae, and mammalian cells. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Agrobacterium Protocols, Second Edition, Volumes 1 and 2 offers plant biotechnologists a gold standard collection of Agrobacterium-mediated transformation techniques for state-of-the-art plant genetic engineering, functional genomic analysis, and crop improvement, and for inspiration in developing new methods for other related and non-related plants.
650		0	_aLife sciences.
650		0	_aPlant science.
650		0	_aBotany.
650	1	4	_aLife Sciences.
650	2	4	_aPlant Sciences.
655		7	_aElectronic books. _2local
700	1		_aWang, Kan. _eeditor.
710	2		_aSpringerLink (Online service)
773	0		_tSpringer eBooks
776	0	8	_iPrinted edition: _z9781588295361
830		0	_aMethods in Molecular Biology, _x1064-3745 ; _v343
856	4	0	_uhttp://ezproxy.alfaisal.edu/login?url=http://dx.doi.org/10.1385/1597451304
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