000			04743nam a22005055i 4500
001			978-1-62703-360-2
003			DE-He213
005			20160615110152.0
007			cr nn 008mamaa
008			130427s2013 xxu| s |||| 0|eng d
020			_a9781627033602 _9978-1-62703-360-2
024	7		_a10.1007/978-1-62703-360-2 _2doi
049			_aAlfaisal Main Library
050		4	_aQD431-431.7
072		7	_aPSBC _2bicssc
072		7	_aSCI007000 _2bisacsh
082	0	4	_a572.6 _223
245	1	0	_aProteomics for Biomarker Discovery _h[electronic resource] / _cedited by Ming Zhou, Timothy Veenstra.
264		1	_aTotowa, NJ : _bHumana Press : _bImprint: Humana Press, _c2013.
300			_aXI, 320 p. 57 illus., 34 illus. in color. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
490	1		_aMethods in Molecular Biology, Methods and Protocols, _x1064-3745 ; _v1002
505	0		_aAffinity Depletion of Plasma and Serum for Mass Spectrometry-Based Proteome Analysis -- Tissue Sample Preparation for Biomarker Discovery -- Subcellular Fractionation for Identification of Biomarkers: Serial Detergent Extraction by Subcellular Accessibility and Solubility -- Analysis of Secreted Proteins -- Preparation of Human Cerebrospinal Fluid for Proteomics Biomarker Analysis -- Proteomic Analysis of Frozen Tissue Samples Using Laser Capture Microdissection -- Use of Formalin Fixed, Paraffin Embedded Tissue for Proteomic Biomarker Discovery -- Phosphopeptide Enrichment Using Offline Titanium Dioxide Columns for Phosphoproteomics -- iTRAQ-Labeling for Biomarker Discovery -- Analysis of Glycoproteins for Biomarker Discovery -- SILAC in Biomarker Discovery -- Trypsin-mediated 18O/16O Labeling for Biomarker Discovery -- Two-dimensional SDS-PAGE Fractionation of Biological Samples for Biomarker Discovery -- Informatics of Protein and Post-Translational Modification Detection via Shotgun Proteomics -- Quantitation of C-Met Tyrosine Phosphorylation Using MRM-MS -- Preparation of Human Serum for Prolactin Measurement by Multiple Reaction Monitoring Mass Spectrometry -- Label-Free Quantitative Shotgun Proteomics Using Normalized Spectral Abundance Factors -- Employment of Complementary Dissociation Techniques for Body Fluid Characterization and Biomarker Discovery -- DPhosphopeptide Microarrays for Comparative Proteomic Profiling of Cellular Lysates -- Tissue Preparation for MALDI MS Imaging of Protein and Peptides -- Plant Proteogenomics: From Protein Extraction to Improved Gene Predictions -- Label-free Differential Analysis of Murine Postsynaptic Densities -- Fractionation of Peptides by Strong Cation Exchange Liquid Chromatography.
520			_aWith the advent of proteomics came the development of technologies, primarily mass spectrometry, which allowed high-throughput identification of proteins in complex mixtures.  While the mass spectrometer resides at the heart of proteomics, its ability to characterize biological samples is only as good as the sample preparation and data analysis tools used in any study.  In Proteomics for Biomarker Discovery, expert researchers in the field detail many of the methods which are now commonly used to study proteomics. These methods and techniques include both label-free approaches and those that utilize stable isotopes incorporated both during cell growth or added via a chemical reaction once the proteome is extracted from the cell. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.     Authoritative and practical, Proteomics for Biomarker Discovery seeks to aid scientists in the further study of the different sample preparation and data analysis tools used in proteomics today.
650		0	_aLife sciences.
650		0	_aProteomics.
650		0	_aProteins.
650	1	4	_aLife Sciences.
650	2	4	_aProtein Science.
650	2	4	_aProteomics.
655		7	_aElectronic books. _2local
700	1		_aZhou, Ming. _eeditor.
700	1		_aVeenstra, Timothy. _eeditor.
710	2		_aSpringerLink (Online service)
773	0		_tSpringer eBooks
776	0	8	_iPrinted edition: _z9781627033596
830		0	_aMethods in Molecular Biology, Methods and Protocols, _x1064-3745 ; _v1002
856	4	0	_uhttp://ezproxy.alfaisal.edu/login?url=http://dx.doi.org/10.1007/978-1-62703-360-2
912			_aZDB-2-PRO
942			_2lcc _cEBOOKS
999			_c277180 _d277180