000 06243cam a2200709Ia 4500
001 ocn298323738
003 OCoLC
005 20180529114302.0
008 090114s1999 cauaf ob 001 0 eng d
019 _a301816706
_a646758431
_a742292041
_a808732579
_a815687266
_a823116753
_a823837905
_a823906085
_a824095725
_a824148244
_a906328537
020 _a9780080859590
_q(electronic bk.)
020 _a0080859593
_q(electronic bk.)
020 _a0125441630
_q(electronic bk.)
020 _a9780125441636
_q(electronic bk.)
020 _a1281755583
020 _a9781281755582
035 _a(OCoLC)298323738
040 _aDLC
_beng
_cDLC
_dAU
049 _aAlfaisal Main Library
050 4 _aQH605.2
_b.M54 1999eb
060 4 _aW1
_bME9615D v.61 1999
060 4 _aQH 605
_bM6835 1999
245 0 0 _aMitosis and meiosis /
_cedited by Conly L. Rieder.
260 _aSan Diego :
_bAcademic Press,
_c©1999.
300 _a1 online resource (xv, 489 pages, [12] pages of plates) :
_billustrations (some color).
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
490 1 _aMethods in cell biology,
_x0091-679X ;
_vv. 61
504 _aIncludes bibliographical references (pages 464-472) and index.
588 0 _aPrint version record.
520 _aMitosis and Meiosis details the wide variety of methods currently used to study how cells divide as yeast and insect spermatocytes, higher plants, and sea urchin zygotes. With chapters covering micromanipulation of chromosomes and making, expressing, and imaging GFP-fusion proteins, this volume contains state-of-the-art "how to" secrets that allow researchers to obtain novel information on the biology of centrosomes and kinetochores and how these organelles interact to form the spindle. Chapters Contain Information On: * How to generate, screen, and study mutants of mitosis in yeast, fungi, and flies * Techniques to best image fluorescent and nonfluorescent tagged dividing cells * The use and action of mitoclastic drugs * How to generate antibodies to mitotic components and inject them into cells * Methods that can also be used to obtain information on cellular processes in nondividing cells.
505 0 _aFront Cover; Methods in Cell Biology, Volume 61; Copyright Page; Contents; Contributors; Preface; Chapter 1. Isolation of Centrosomes from Drosophila Embryos; I. Introduction; II. Isolation of Centrosomes from Drosophila Embryos; III. Assays for Microtubule Nucleation by Isolated Centrosomes; IV. Conclusions; References; Chapter 2. Studying the Composition and Function of Centrosomes in Vertebrates; I. Introduction; II. Isolation of Centrosomes from Animal Cells; III. Preparation of Immunological Probes from Isolated Centrosomes; IV. Ultrastructural Analysis of Isolated Centrosomes.
505 8 _aV. Biochemical Composition of CentrosomesVI. Functional Assays of Isolated Centrosomes; VII. Prospects; References; Chapter 3. Isolation of Centrosomes from Spisulu solidissima Oocytes; I. Introduction; II. Obtaining Organisms; III. Isolation and Activation of Spisulu solidissima Oocytes; IV. Preparation of Oocyte Lysates; V. Preparation of Microtubule Protein; VI. Isolation of Centrosomes from Activated Oocyte Lysates; VII. lmmunofluorescence of Centrosomes and Asters; VIII. Electron Microscopy of Asters and Centrosomes; IX. Summary; References.
505 8 _aChapter 4. Methods for in Situ Localization of Proteins and DNA in the Centromere-Kinetochore ComplexI. Introduction; II. In Situ Localization of Proteins: Indirect Immunofluorescence; III. In Situ Localization of Proteins: Immunogold EM; IV. Fluorescent in Situ Hybridization Using DNA Satellite Probes; V. Combination Staining: DNA/Protein; VI. Specialized Techniques; References; Chapter 5. Three-Dimensional Transmission Electron Microscopy and Its Application to Mitosis Research; I. Introduction; II. Resolution and Choosing between Tomography and Serial Sections; III. Electron Tomography.
505 8 _aIV. Serial Section ReconstructionV. Analysis and Display of 3D Reconstructions; VI. Software Packages; VII. Summary and Conclusions; References; Chapter 6. Enlightening Mitosis: Construction and Expression of Green Fluorescent Protein Fusion Proteins; I. Introduction: Visualizing the Molecular Anatomy of the Spindle; II. Fluorescence Properties of GFP; III. Strategies for Constructing Fusion Proteins; IV. Expression in Mammalian Cells; References; Chapter 7. Recombinant p50/Dynamitin as a Tool to Examine the Role of Dynactin in Intracellular Processes; I. Introduction.
505 8 _aII. Production of Recombinant p50/DynamitinIII. Disruption of the Dynactin Complex by p50/Dynamitin in Xenopus Egg Extracts; IV. Disruption of Spindle Poles Using p50/Dynamitin; References; Chapter 8. In Vitro Assays for Studying Saccharomyces cerevisiae Kinetochore Activity; I. Introduction; II. Microtubule-Binding Assays for S. cerevisiae Kinetochores; Ill. Band Shift Assay for the Kinetochore Complex; References; Chapter 9. Fluorescent Speckle Microscopy of Spindle Microtubule Assembly and Motility in Living Cells; I. lntroduction.
590 _aElsevier
_bScienceDirect All Books
650 0 _aCell division.
650 0 _aMitosis.
650 0 _aMeiosis.
650 1 2 _aCell Division.
650 2 2 _aCell Fractionation
_xmethods.
650 2 2 _aMeiosis.
650 2 2 _aMitosis.
650 6 _aMitose.
650 6 _aMéiose.
650 7 _aSCIENCE
_xLife Sciences
_xDevelopmental Biology.
_2bisacsh
650 7 _aCell division.
_2fast
_0(OCoLC)fst00850186
650 7 _aMeiosis.
_2fast
_0(OCoLC)fst01015610
650 7 _aMitosis.
_2fast
_0(OCoLC)fst01024031
650 1 7 _aCeldeling.
_2gtt
650 1 7 _aMeiose.
_2gtt
650 7 _aCitologia.
_2larpcal
655 7 _aElectronic books.
_2local
700 1 _aRieder, Conly L.
710 2 _aScienceDirect eBooks.
776 0 8 _iPrint version:
_tMitosis and meiosis.
_dSan Diego : Academic Press, ©1999
_z9780125441636
_z0125441630
_w(DLC) 99172833
_w(OCoLC)40513158
830 0 _aMethods in cell biology ;
_vv. 61.
856 4 0 _uhttp://ezproxy.alfaisal.edu/login?url=https://www.sciencedirect.com/science/bookseries/0091679X/61
942 _2lcc
_cEBOOKS
264 _c©1999.
999 _c527118
_d527118