000 | 06243cam a2200709Ia 4500 | ||
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001 | ocn298323738 | ||
003 | OCoLC | ||
005 | 20180529114302.0 | ||
008 | 090114s1999 cauaf ob 001 0 eng d | ||
019 |
_a301816706 _a646758431 _a742292041 _a808732579 _a815687266 _a823116753 _a823837905 _a823906085 _a824095725 _a824148244 _a906328537 |
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020 |
_a9780080859590 _q(electronic bk.) |
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020 |
_a0080859593 _q(electronic bk.) |
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020 |
_a0125441630 _q(electronic bk.) |
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020 |
_a9780125441636 _q(electronic bk.) |
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020 | _a1281755583 | ||
020 | _a9781281755582 | ||
035 | _a(OCoLC)298323738 | ||
040 |
_aDLC _beng _cDLC _dAU |
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049 | _aAlfaisal Main Library | ||
050 | 4 |
_aQH605.2 _b.M54 1999eb |
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060 | 4 |
_aW1 _bME9615D v.61 1999 |
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060 | 4 |
_aQH 605 _bM6835 1999 |
|
245 | 0 | 0 |
_aMitosis and meiosis / _cedited by Conly L. Rieder. |
260 |
_aSan Diego : _bAcademic Press, _c©1999. |
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300 |
_a1 online resource (xv, 489 pages, [12] pages of plates) : _billustrations (some color). |
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336 |
_atext _btxt _2rdacontent |
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337 |
_acomputer _bc _2rdamedia |
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338 |
_aonline resource _bcr _2rdacarrier |
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490 | 1 |
_aMethods in cell biology, _x0091-679X ; _vv. 61 |
|
504 | _aIncludes bibliographical references (pages 464-472) and index. | ||
588 | 0 | _aPrint version record. | |
520 | _aMitosis and Meiosis details the wide variety of methods currently used to study how cells divide as yeast and insect spermatocytes, higher plants, and sea urchin zygotes. With chapters covering micromanipulation of chromosomes and making, expressing, and imaging GFP-fusion proteins, this volume contains state-of-the-art "how to" secrets that allow researchers to obtain novel information on the biology of centrosomes and kinetochores and how these organelles interact to form the spindle. Chapters Contain Information On: * How to generate, screen, and study mutants of mitosis in yeast, fungi, and flies * Techniques to best image fluorescent and nonfluorescent tagged dividing cells * The use and action of mitoclastic drugs * How to generate antibodies to mitotic components and inject them into cells * Methods that can also be used to obtain information on cellular processes in nondividing cells. | ||
505 | 0 | _aFront Cover; Methods in Cell Biology, Volume 61; Copyright Page; Contents; Contributors; Preface; Chapter 1. Isolation of Centrosomes from Drosophila Embryos; I. Introduction; II. Isolation of Centrosomes from Drosophila Embryos; III. Assays for Microtubule Nucleation by Isolated Centrosomes; IV. Conclusions; References; Chapter 2. Studying the Composition and Function of Centrosomes in Vertebrates; I. Introduction; II. Isolation of Centrosomes from Animal Cells; III. Preparation of Immunological Probes from Isolated Centrosomes; IV. Ultrastructural Analysis of Isolated Centrosomes. | |
505 | 8 | _aV. Biochemical Composition of CentrosomesVI. Functional Assays of Isolated Centrosomes; VII. Prospects; References; Chapter 3. Isolation of Centrosomes from Spisulu solidissima Oocytes; I. Introduction; II. Obtaining Organisms; III. Isolation and Activation of Spisulu solidissima Oocytes; IV. Preparation of Oocyte Lysates; V. Preparation of Microtubule Protein; VI. Isolation of Centrosomes from Activated Oocyte Lysates; VII. lmmunofluorescence of Centrosomes and Asters; VIII. Electron Microscopy of Asters and Centrosomes; IX. Summary; References. | |
505 | 8 | _aChapter 4. Methods for in Situ Localization of Proteins and DNA in the Centromere-Kinetochore ComplexI. Introduction; II. In Situ Localization of Proteins: Indirect Immunofluorescence; III. In Situ Localization of Proteins: Immunogold EM; IV. Fluorescent in Situ Hybridization Using DNA Satellite Probes; V. Combination Staining: DNA/Protein; VI. Specialized Techniques; References; Chapter 5. Three-Dimensional Transmission Electron Microscopy and Its Application to Mitosis Research; I. Introduction; II. Resolution and Choosing between Tomography and Serial Sections; III. Electron Tomography. | |
505 | 8 | _aIV. Serial Section ReconstructionV. Analysis and Display of 3D Reconstructions; VI. Software Packages; VII. Summary and Conclusions; References; Chapter 6. Enlightening Mitosis: Construction and Expression of Green Fluorescent Protein Fusion Proteins; I. Introduction: Visualizing the Molecular Anatomy of the Spindle; II. Fluorescence Properties of GFP; III. Strategies for Constructing Fusion Proteins; IV. Expression in Mammalian Cells; References; Chapter 7. Recombinant p50/Dynamitin as a Tool to Examine the Role of Dynactin in Intracellular Processes; I. Introduction. | |
505 | 8 | _aII. Production of Recombinant p50/DynamitinIII. Disruption of the Dynactin Complex by p50/Dynamitin in Xenopus Egg Extracts; IV. Disruption of Spindle Poles Using p50/Dynamitin; References; Chapter 8. In Vitro Assays for Studying Saccharomyces cerevisiae Kinetochore Activity; I. Introduction; II. Microtubule-Binding Assays for S. cerevisiae Kinetochores; Ill. Band Shift Assay for the Kinetochore Complex; References; Chapter 9. Fluorescent Speckle Microscopy of Spindle Microtubule Assembly and Motility in Living Cells; I. lntroduction. | |
590 |
_aElsevier _bScienceDirect All Books |
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650 | 0 | _aCell division. | |
650 | 0 | _aMitosis. | |
650 | 0 | _aMeiosis. | |
650 | 1 | 2 | _aCell Division. |
650 | 2 | 2 |
_aCell Fractionation _xmethods. |
650 | 2 | 2 | _aMeiosis. |
650 | 2 | 2 | _aMitosis. |
650 | 6 | _aMitose. | |
650 | 6 | _aMéiose. | |
650 | 7 |
_aSCIENCE _xLife Sciences _xDevelopmental Biology. _2bisacsh |
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650 | 7 |
_aCell division. _2fast _0(OCoLC)fst00850186 |
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650 | 7 |
_aMeiosis. _2fast _0(OCoLC)fst01015610 |
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650 | 7 |
_aMitosis. _2fast _0(OCoLC)fst01024031 |
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650 | 1 | 7 |
_aCeldeling. _2gtt |
650 | 1 | 7 |
_aMeiose. _2gtt |
650 | 7 |
_aCitologia. _2larpcal |
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655 | 7 |
_aElectronic books. _2local |
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700 | 1 | _aRieder, Conly L. | |
710 | 2 | _aScienceDirect eBooks. | |
776 | 0 | 8 |
_iPrint version: _tMitosis and meiosis. _dSan Diego : Academic Press, ©1999 _z9780125441636 _z0125441630 _w(DLC) 99172833 _w(OCoLC)40513158 |
830 | 0 |
_aMethods in cell biology ; _vv. 61. |
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856 | 4 | 0 | _uhttp://ezproxy.alfaisal.edu/login?url=https://www.sciencedirect.com/science/bookseries/0091679X/61 |
942 |
_2lcc _cEBOOKS |
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264 | _c©1999. | ||
999 |
_c527118 _d527118 |